dextran hydrodynamic radius

dextran hydrodynamic radius

Thus, it is a common practice to express the frictional coefficient of rigid structures as. (2003). principles of dynamic light scattering These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. government site. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Carbohydrate Polymers, 74, 372-378. Characterisation of insulin analogues therapeutically available to Table 2 compares the properties of nanoparticles coated with one or other polymer. Catiker, E.; Guner, A. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol ; Ross-Murphy, S.B. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Drug deliv. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence (2003). We observe that this maximum radius is below (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. TEWL measurements were taken 30min after microporation of the human and pig skin. Journal Argentine Chemical Society 100 (2013) 25-34. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Macromolecules. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence Pure Appl. Bohdanecky, Miloslav (1996). Unperturbed dimensions and theta temperature of dextran in aqueous solutions. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free It is well tolerated on parenteral infusion by most people but a very low incidence Provided by the Springer Nature SharedIt content-sharing initiative. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Radiofrequency ablation of malignant liver tumors. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). 2013;3(5):3588605. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. NCI CPTC Antibody Characterization Program. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Corp. (Seoul, Korea). Viscotek GmbH, Europe. ; Grishchenko, A.E. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. This conjecture found to be correct. Garcia de la Torre, J.; Carrasco, B. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Porcine wound models for skin substitution and burn treatment. Journal Non-Newtonian Fluid Mech., 97, 295-301. II. Drug Discov Today Technol. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). ; Aberle, T; Burchard, W. (2000). The above data are not useful because it can not be compared with those obtained in this work. PMC ACS Nano. The authors declare that they have no competing interests. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. However, in vitro investigations are very few. Amersham Biosciences, AA Edition, www.amershambiosciences.com. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. Bahary, W.S. Format), Citation-(BibTeX Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Prausnitz MR, Langer R. Transdermal drug delivery. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Carbohydrate Polymers 88 1440-1444. (2001). Nordmeier, Eckliard (1993). The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. 43-1, 115-147. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Synthesis and characterization of blue dextrans Curley SA. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Biomaterials. Eremeeva, T. E.; Bykova, T. O. Physicochemical studies of oligodextran. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. hydrodynamic 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. dextran hydrodynamic Dilute Solution. The Mark-Houwink a constant is close to 0.5 in poor solvents. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. Dextran produced from Weissella cibaria JAG8 was purified and characterized. Principles of Size Exclusion Chromatography SEC (GFC Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Global hydrodynamic analysis of the molecular flexibility of galactomannans. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. (2008). The lower molecular weight of FITC-dextran permeated more on both human and pig skin. dextran Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. 1984;17(6):11703. Food Funct. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). HSE and DK helped to porate the skin and to measure TEWL. where tr is the column retention time in minutes (2 of 0.9792). Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. On single point determination of intrinsic viscosity. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. hydrodynamic The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Pathan IB, Setty CM. Ibrahim O, Munavalli GS, Dover JS. Bookshelf Terms and Conditions, Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Chem. (1954). Data File Dextran. (2004). Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran.

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